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| Cat. Number | SYBR Green I染料，用于實時PCR dsGreen for Real-Time PCR, 100× |
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| Chemical Name | SYBR Green I染料，用于實時PCR dsGreen for Real-Time PCR, 100× |
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| Qty 1 |
1ml |
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| Appearance | light orange solution |
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| Storage condition | -20°C |
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| References | dsGreen, an analog of SYBR? Green I, is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allow to use dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen – unlabeled primers are sufficient. Unlike other preparations of dsGreen provided by Lumiprobe for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:
Recommended protocolRealtime qPCR with dsGreen or SYBR Green I Note: fluorescent properties of dsGreen bound to dsDNA below are taken from the following publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103 Spectral properties
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